Job descriptions for Novick lab 1. Post-doctoral. We are looking for an individual who has training and experience in microbiology and bacterial molecular genetics. The research project on which he/she will work is outlined in our recent paper: Ram, et al, Nat Biotechnol. 2018 Nov;36(10):971-976, Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice, as described in the following abstract, Staphylococcus aureus and other staphylococci continue to cause life-threatening infections in both hospital and community settings. They have become increasingly resistant to antibiotics, especially β-lactams and aminoglycosides, and their infections are now, in many cases, untreatable. Here we present a non-antibiotic, non-phage method of treating staphylococcal infections by engineering of the highly mobile staphylococcal pathogenicity islands (SaPIs). We replaced the SaPIs’ toxin genes with antibacterial cargos to generate antibacterial drones (ABDs) that target the infecting bacteria in the animal host, express their cargo, kill or disarm the bacteria and thus abrogate the infection. Here we have constructed ABDs with either a CRISPR-Cas9 bactericidal or a CRISPR-dCas9 virulence-blocking module. We show that both ABDs block the development of a murine subcutaneous S. aureus abscess and that the bactericidal module rescues mice given a lethal dose of S. aureus intraperitoneally. Experience with staphylococcus will be highly advantageous for this position 2. Lab technician. We are looking for an individual who has training in microbiology and is able to perform standard microbiological procedures – culturing organisms, genetic transduction and transformation, isolating mutants, etc. Experience with mice will be highly advantageous. While the job will involve experimentation, it will also involve standard lab management – ordering supplies and media, maintaining equipment, etc.
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